The strike, called by the Filt Cigl union, will be held between 11am and 3pm.
“The strike, the first in many years, is related to the living and working conditions of those who have been affected by previous business decisions,” Nino Cortorillo, the union’s secretary, said.
“Working hours have risen while the procedure used for redundancies, in our opinion, is outside of the norms.”
Alitalia has been struggling to turn its fortunes around since a 49 percent stake in the company was acquired by the Abu Dhabi-based carrier, Etihad, in 2014.
Australian Cramer Ball, who was appointed Alitalia CEO in March, told employees that the strike was “incomprehensible”.
In a letter seen by Ansa, he said: “It is incomprehensible that unions are ready to damage our clients and affect Alitalia’s business to defend the privileges of a part of employees”.
The company said in a statement that it has extended the period of rebooking tickets up to 29th October 2016 “in order to minimise as much as possible the inconvenience to passengers”.